How To Create Real-Time PCR Primers Using Primer-BLAST
This video tutorial is part of the Mastering qPCR Online Course in The Top Tip Bio Academy.
🎓 MASTERING QPCR ONLINE COURSE 🎓
https://courses.toptipbio.com/p/maste...
In this video tutorial, I will show you how to create real-time PCR primers by using NCBI's Primer-BLAST free online software.
The Primer-BLAST software is great since it uses the Primer3 algorithm to design optimal primers, while simultaneously blasting these sequences on the genome. This ensures the primers will not amplify off-target regions.
THE ONLINE GUIDE
http://toptipbio.com/real-time-pcr-pr...
NCBI WEBSITE
https://www.ncbi.nlm.nih.gov/
VIDEO BREAKDOWN
Step 1: Finding your gene variant (00:18)
Step 2: Loading Primer-BLAST (01:53)
Step 3: The Primer-BLAST settings (02:22)
Step 4: The primer reports (08:40)
MORE HELPFUL HINTS & TIPS
http://toptipbio.com/
FOLLOW US
Facebook: / toptipbio
Twitter: / toptipbio
LinkedIn: / top-tip-bio
Watch video How To Create Real-Time PCR Primers Using Primer-BLAST online, duration hours minute second in high quality that is uploaded to the channel Steven Bradburn 14 February 2018. Share the link to the video on social media so that your subscribers and friends will also watch this video. This video clip has been viewed 143,227 times and liked it 2.5 thousand visitors.
