How To Perform The Delta-Delta Ct Method (In Excel)
This video tutorial is part of the Mastering qPCR Online Course in The Top Tip Bio Academy.
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In this video tutorial, I will show you how to perform the delta-delta Ct method by using Microsoft Excel.
The delta-delta Ct method is a simple formula used in order to calculate the relative fold gene expression of samples when performing real-time polymerase chain reaction (also known as qPCR).
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VIDEO BREAKDOWN
Step 1: Average the Ct values (00:53)
Step 2: Calculate delta Ct (01:59)
Step 3: Calculate the average delta Ct for controls (02:46)
Step 4: Calculate delta-delta Ct (03:39)
Step 5: Calculate 2^-(delta-delta Ct) (04:54)
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Watch video How To Perform The Delta-Delta Ct Method (In Excel) online, duration hours minute second in high quality that is uploaded to the channel Steven Bradburn 09 March 2018. Share the link to the video on social media so that your subscribers and friends will also watch this video. This video clip has been viewed 298,470 times and liked it 3.3 thousand visitors.
